https://doi.org/10.21203/rs.3.rs-5012240/v1
Journal: 2024
Publisher: Springer Science and Business Media LLC
Authors:
- Feiran Xu
- Juan Tang
- Tong Ji
- Yu Wang
- Xuan Tao
- Zheng Xiong
- Bao Zhang
- Zhigao Wang
Abstract
<title>Abstract</title> Current pharmacological treatments have not been effective in achieving sustainable and long-term weight loss. Studies have shown that reducing dietary fat alone is not sufficient to prevent weight gain and obesity. Proteins are used to change diet and cause weight loss. Rapeseed plant protein is a high-quality protein, and RPI provides all essential amino acids needed for proper nutrition. However, the suitability of RPI as a dietary protein source for weight loss and its potential effects on plasma amino acid levels in lower floating cells and the induction of satiety in lower floating cells remains unknown. Therefore, we analyzed the amino acid composition of rapeseed protein isolate (RPI) and evaluated its effect on high-fat diet-induced obesity using <italic>in vivo</italic> and <italic>in vitro</italic> models. The RPI group exhibited elevated levels of anti-obesity amino acids in the plasma compared to the CS and SPI groups, leading to reduced food intake, weight gain, Lee index, white adipose tissue weight, improved serum lipid profiles, and beneficial alterations in the intestinal microbiota of obese rats. Molecular analyses elucidated RPI’s role in metabolic signaling, revealing its activation of Mammalian Target of Rapamycin/p70S6K (mTOR/p70S6K) pathway, inhibition of AMP-activated protein kinase (AMPK) and Acetyl-CoA carboxylase (ACC) phosphorylation, and modulation of the melanocortin system by increasing α-Melanocyte-Stimulating Hormone (α-MSH) and Melanocortin 4 Receptor (MC4R) levels in paraventricular nucleus neurons. These findings suggest that RPI could potentially reduce obesity through amino acid-mediated inhibition, appetite suppression, and increased metabolism.
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About this publication
Publication type | Оприлюднений вміст |
Number of citations | 0 |
Number of works in the list of references | 31 |
Journal indexed in Scopus | No |
Journal indexed in Web of Science | No |